Search | Engineering

Subscribe Submit

Home Journals Focus Achievement Fronts About Us 中文版

Resource Type

Journal Article 3

Year

2023 1

2022 2

Keywords

organoids 2

3D culture 1

Biofabrication 1

Kidney 1

Organoids 1

Tissue engineering 1

Vascularization 1

cholangiocytes 1

decellularized extracellular matrix 1

hepatocytes 1

liver regeneration 1

regulatory mechanisms 1

stem cells 1

tissue repair 1

transplantation/grafting strategies 1

open ︾

Search scope:

排序： Display mode:

Decellularized extracellular matrix mediates tissue construction and regeneration

Frontiers of Medicine 2022, Volume 16, Issue 1, Pages 56-82 doi: 10.1007/s11684-021-0900-3

Abstract: Contributing to organ formation and tissue regeneration, extracellular matrix (ECM) constituents provide tissue with three-dimensional (3D) structural integrity and cellular-function regulation. Containing the crucial traits of the cellular microenvironment, ECM substitutes mediate cell–matrix interactions to prompt stem-cell proliferation and differentiation for 3D organoid construction in vitro or tissue regeneration in vivo. However, these ECMs are often applied generically and have yet to be extensively developed for specific cell types in 3D cultures. Cultured cells also produce rich ECM, particularly stromal cells. Cellular ECM improves 3D culture development in vitro and tissue remodeling during wound healing after implantation into the host as well. Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select, produce, and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration. Overall, the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed. Moreover, current preclinical applications by which ECM components modulate the wound-healing process are reviewed.

Keywords： decellularized extracellular matrix 3D culture organoids tissue repair

HTML PDF Collect

Liver cell therapies: cellular sources and grafting strategies

Frontiers of Medicine 2023, Volume 17, Issue 3, Pages 432-457 doi: 10.1007/s11684-023-1002-1

Abstract: The liver has a complex cellular composition and a remarkable regenerative capacity. The primary cell types in the liver are two parenchymal cell populations, hepatocytes and cholangiocytes, that perform most of the functions of the liver and that are helped through interactions with non-parenchymal cell types comprising stellate cells, endothelia and various hemopoietic cell populations. The regulation of the cells in the liver is mediated by an insoluble complex of proteins and carbohydrates, the extracellular matrix, working synergistically with soluble paracrine and systemic signals. In recent years, with the rapid development of genetic sequencing technologies, research on the liver’s cellular composition and its regulatory mechanisms during various conditions has been extensively explored. Meanwhile breakthroughs in strategies for cell transplantation are enabling a future in which there can be a rescue of patients with end-stage liver diseases, offering potential solutions to the chronic shortage of livers and alternatives to liver transplantation. This review will focus on the cellular mechanisms of liver homeostasis and how to select ideal sources of cells to be transplanted to achieve liver regeneration and repair. Recent advances are summarized for promoting the treatment of end-stage liver diseases by forms of cell transplantation that now include grafting strategies.

Keywords： liver regeneration hepatocytes cholangiocytes stem cells organoids regulatory mechanisms transplantation